Journal of Coloproctology Journal of Coloproctology
J Coloproctol. (Rio J.) 2018;38:1-8 - Vol. 38 Num.1 DOI: 10.1016/j.jcol.2017.08.005
Original Article
The role of Phosphatidylinositol 3 kinase (PI3K) and Cycloxygenase-2 (COX2) in carcinogenesis of colorectal polyps
O papel de PI3K e COX2 na carcinogênese de pólipos colorretais
Raul Alberto Anselmi Júniora, Cleber Machado de Souzab, Marina Luise Viola de Azevedob, Mário Rodrigues Montemor Nettoc, Rosimeri Kuhl Svoboda Baldind,, , Ana Paula Martins Sebastiãod, Luiz Felipe Paula Soarese, Luis Fernando Tullioe, Lúcia de Noronhaa
a Pontifícia Universidade Católica do Paraná (PUCPR), Faculdade de Medicina, Curitiba, PR, Brazil
b Pontifícia Universidade Católica do Paraná (PUCPR), Escola de Saúde e Biociências, Curitiba, PR, Brazil
c Universidade Estadual de Ponta Grossa (UEPG), Ponta Grossa, PR, Brazil
d Universidade Federal do Paraná (UFPR), Departamento de Patologia Médica, Curitiba, PR, Brazil
e Hospital Santa Cruz, Serviço de Endoscopia, Curitiba, PR, Brazil
Received 11 July 2017, Accepted 26 August 2017
Abstract
Objectives

Determine immunohistochemical expression of Phosphatase and tensin homolog (PTEN), Phosphatidylinositol 3 kinase (PI3K), Cycloxygenase-2 (COX2) and one proliferation marker (Ki67) in colorectal polyps and correlate with clinical and pathological data in search of carcinogenic pathways.

Methods

The reports of 297 polyps diagnosed through endoscopy were reviewed for parameters including age, gender, prior colorectal cancer, the presence of multiple polyps, and polyps’ location, appearance and size. Was conducted a microscopic morphometric computerized analysis of immunohistochemical expression using, the selected antibodies and correlated with clinical and pathological variables.

Results

The tissue immunohistochemical expression was higher in right colon polyps for the proliferation marker and Phosphatidylinositol 3 kinase (p0.0001 and 0.057 respectively). Cycloxygenase-2 and Phosphatase and tensin homolog demonstrated higher tissue immunoexpression in pedunculated polyps (p=0.009 and 0.002 respectively). Cycloxygenase-2 exhibited higher immunoexpression in larger polyps (p=0.005). Phosphatidylinositol 3 kinase, Cycloxygenase-2, Phosphatase and tensin homolog and the proliferation marker exhibited higher immunoexpression in high-grade dysplastic polyps (p=0.031, 0.013, 0.044 and <0.001 respectively). Phosphatase and tensin homolog labeling was higher in polyps with high-grade dysplasia and lower in some of serrated lesions (p=0.044).

Conclusions

The greater expression of the proliferation marker and Phosphatidylinositol 3 kinase in the right colon may be related to right-sided colorectal carcinogenesis. The proliferation marker, Cycloxygenase-2 and Phosphatidylinositol 3 kinase results can be associated with progression of polyps to colorectal cancer. The higher Phosphatase and tensin homolog expression suggests its attempt to control the cell cycle.

Resumo
Objetivos

Determinar a expressão imuno-histoquímica de Fosfatase homóloga a tensina (PTEN), Fosfatidilinositol-3-cinase (PI3K), Ciclooxigenase-2 (COX2) e um marcador de proliferação (Ki67) em pólipos colorretais e correlacionar com dados clínicos e patológicos buscando sua correspondência na carcinogênese.

Métodos

Revisados 297 pólipos diagnosticados através de endoscopia quanto a idade, gênero, história de câncer colorretal, número, localização, aparência e tamanho dos pólipos. Realizadas as avaliações morfométricas computadorizadas das expressões imuno-histoquímicas dos marcadores selecionados, que foram correlacionadas com variáveis clínicas e patológicas.

Resultados

A expressão do marcador de proliferação e da Fosfatidilinositol-3-cinase foi maior nos pólipos do cólon direito (p=<0,0001 e 0.057 respectivamente). Ciclooxigenase-2 e Fosfatase homóloga a tensina demonstraram maior imunoexpressão em pólipos pediculados (p=0,009 e 0,002, respectivamente). Ciclooxigenase-2 expressou mais em pólipos maiores (p=0,005). Fosfatidilinositol-3-cinase, Ciclooxigenase-2, Fosfatase homóloga a tensina e o marcador de proliferação expressaram mais em pólipos com displasia de alto grau (p=0,031, 0,013, 0,044 e <0,001, respectivamente). Fosfatase homóloga a tensina marcou mais pólipos com displasia de alto grau que lesões serrilhadas (p=0,044).

Conclusões

A maior expressão do marcador de proliferação e Fosfatidilinositol-3-cinase à direita pode estar relacionada à carcinogênese do lado direito do cólon. Os resultados do marcador de proliferação, Ciclooxigenase-2 e Fosfatidilinositol-3-cinase podem ser associados à progressão dos pólipos para câncer. A expressão aumentada de Fosfatase homóloga a tensina sugere tentativa de controle do ciclo celular.

Keywords
PI3K, COX2, Colorectal cancer, Adenoma
Palavras-chave
PI3K, COX2, Câncer colorretal, Adenoma
Introduction

Colorectal cancer is the third most frequently diagnosed malignancy in men and the second most frequently diagnosed cancer type in women worldwide, with 1.3 million new case reports and 694,000 deaths in 2012.1–4

Colorectal carcinogenesis phenomena may be studied through the evaluation of signaling pathway protein expression as these events may be the result of alterations in genes that are coding proteins of cycle cell.5–7 Thus, the changes in theses proteins’ structure may modify functional aspects involving these molecules.

It has already been well-characterized three molecular pathways of colorectal carcinogenesis: chromosomal instability, microsatellite instability and serrated pathway.6

The pathway of chromosomal instability, also called the classical pathway, occurs due to somatic or germline mutation. This pathway occurs through a sequence of cumulative molecular changes, denominated “adenoma–carcinoma sequence”.5

Concerning the pathway of microsatellite instability, there is a dysfunction of deoxyribonucleic acid (DNA) repair enzymes, due to germline mutations in mismatch repair genes (MMR), with formation of tens to hundreds of repetitions of short sequences of nucleotide bases by the genome, called microsatellites.8

Regarding the serrated pathway, there is no mutation of MMR genes but an epigenetic phenomenon, called hypermethylation of promoter gene MMR enzyme that makes the silencing of their gene expression.9,10

Apparently, genetic alterations that occur in colorectal cancer follow the histologic progression of adenomas.11 The use of molecular and tissue immunohistochemical markers for colorectal polyp evaluation has been helpful in predicting malignant transformation and has provided a better understanding of this neoplastic biological behavior.12

PTEN (phosphatase and tensin homolog) acts on the negative regulation of the PI3K-AKT-mTOR (phosphatidylinositol 3-kinase – serine/threonine kinase – mechanistic target of rapamycin) pathways and on the silencing of growth factor receptors such as Epidermal growth factor receptor (EGFR), Human Epidermal growth factor Receptor-type 2 (HER-2) and insulin-like growth factor 1 (IGFR). PTEN function loss occurs through mutations, deletions or genetic silencing in a wide range of human cancers. PTEN is the second most mutated tumor suppressor gene after the tumor protein p53 (Tp53) gene.13,14

PI3K promote cellular proliferation and survival. Phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit alpha isoform (PIK3CA) gene mutations have been identified in 10–20% of colorectal cancer case reports, and these mutations may also occur in non-malignant colorectal lesions, demonstrating that these alterations may be significant events in colorectal carcinogenesis.15–17 PI3K pathway activation increases cyclooxygenase-2 (COX2) activity, resulting in the inhibition of apoptosis.18–20 PI3K activity is opposed by PTEN; because its negative regulation is essential to cellular growth control maintenance.21,22

COX2 is responsible for prostaglandin E2 (PGE2) production, which is involved in colorectal tumorigenesis. Some authors have highlighted the importance of PGE2 as an effector cell signaling pathway activator, including the PI3K/AKT pathways.23–27

Ki67 (mouse monoclonal antibody against a nuclear antigen expressed in human cells during G1, S, G2 and M phases of cell cycle) indicates the fraction of tumor growth, signifying the potential of cellular division that a specific group of cells presents at a certain moment.28–30

This study will evaluate the tissue immunohistochemical expression of biomarkers such as PTEN, PI3K, COX2 and Ki67 in benign colorectal polyps, correlating these findings with clinical and pathological data.

MethodsStudy group

297 benign colon polyp formalin-fixed paraffin-embedded (FFPE) samples (adenomatous and non-adenomatous) from 229 consecutive patients undergoing colonoscopy at the Hospital Santa Cruz, Curitiba, Brazil (2008), were evaluated. Patients with inflammatory bowel disease, familial adenomatous polyposis and Lynch Syndrome were excluded. Data such as age, sex, location, presence of multiple polyps, history of previous cancer, polyp size and endoscopic appearance were retrieved from the respective endoscopy reports. Fulguration and fragmentation artifacts and inflammatory polyps were excluded from the analysis. This study was approved by the Ethical Committee in Research at Hospital de Clínicas of Universidade Federal do Paraná and have a Certificate of Presentation for Ethical Appreciation number 34605914.3.0000.0096.

Case review and immunohistochemical methods

Microscopic slides stained with hematoxylin–eosin were reviewed, with samples categorized according to current World Health Organization (WHO) histologic classification of tumors of the digestive system31 (conventional adenomas and serrated polyps), and their dysplasia grades (low or high). All samples were then segregated into six histologic groups: (1) high-grade dysplastic conventional adenomas, including tubular and villous (HDA); (2) low-grade dysplastic conventional adenomas, including tubular and villous (LDA); (3) traditional serrated adenomas (TSA); (4) sessile serrated adenomas (SSA); (5) microvesicular hyperplastic polyps (MVHP) and (6) goblet cell rich hyperplastic polyps (GCHP). No mucin poor hyperplastic polyps (MPHP) were found among studied cases.

Tissue microarrays (TMA) that contained 1 sample per case (3mm diameter) were constructed.32 All TMA slides were stained together with a positive control slide (colon cancer sample) and a negative control slide to which no primary antibody was added.

For immunohistochemistry, the immunoperoxidase assay as reported by some authors32 was used, with modifications. Antigen retrieval was performed using the BioSB®™ immunoretriever (Santa Barbara, CA, USA). The samples were incubated with the secondary antibody (DAKO ADVANCE™ HRP (horseradish peroxidase) SYSTEM, DakoCytomation, Inc., CA, USA) for 30min.32 The antibodies used were rabbit anti-COX2 polyclonal antibody (Spring Bioscience, Pleasanton, CA, USA), 1:200; rabbit anti-human PIK3CA monoclonal antibody (Spring Bioscience, Pleasanton, CA, USA), 1:100; mouse anti-Ki67, Clone MIB-1 (mindbomb E3 ubiquitin protein ligase 1) Dako – Code IR626, Glostrup, Denmark, ready-to-use; and PTEN monoclonal antibody (Novocastra Buffalo Grove, IL, USA), 1:200.

The immunostained slides were observed using an optical microscope Olympus® BX50 (Tokyo, Japan), coupled to a Dinoeye video camera enhanced by image analysis software Image Pro Plus™ (Maryland, USA). For each sample, four photomicrographs were taken in HPF (high power field=400×), with a total area of 115,226.1μm2 and with 1024×768pixels. The positive control HPF photomicrography was chosen as the “mask”, which contained adequate levels of positive tissue immunoexpression signal. The mask was then superimposed to the samples photomicrographs. Based on the ideal positive tissue immunoexpression signal obtained from the mask, the image analysis software Image Pro Plus() identified the positive areas in the samples and is able to transform these results into positive immunoexpression area per square micron (μm2). The area in μm2 obtained with this method was divided by the constant 115,226.1μm2, which is the total area of the HPF observed, thus generating a percentage value for each HPF. For each polyp, an average percentage of positive area was determined in four HPF images. This method was used for PTEN, PIK3CA and COX2 biomarkers.

For Ki67, positive cell numbers were counted for 100 cells per HPF, and the results were reported in %.

The statistical analysis compared the studied biomarkers’ immunohistochemical expression with the previous presence of cancer; presence or absence of multiple polyps; location in the right or left colon; sessile or pedunculated endoscopic appearance; polyp diameter size less than 5mm, between 6 and 10mm and larger than 10mm; and the six histological groups. The results were described using Mann–Whitney and Kruskal–Wallis non-parametric tests. A Receiver Operating Characteristic (ROC) curve was adjusted to evaluate the discrimination power of each variable; p (calculated probability) <0.05 values indicated statistical significance. Data were analyzed using the Statistical Package for the Social Sciences (SPSS) v.20.0 computer program.

Results

The clinical and pathological data were grouped in Table 1.

Table 1.

Clinical, endoscopic and anatomopathological data for colorectal polyps.

Total number of polyps (n=297)  Characteristics  Number of polyps by category
n (%) 
Age of individuals with polyp(s)Minimum: 19 years   
Maximum: 91 years   
Median: 60 years   
GenderPolyps in men  170 (57.2) 
Polyps in women  127 (42.7) 
Previous cancer historyWith no previous cancer  285 (96) 
With previous cancer  12 (4) 
One or multipleOne polyp:  106 (35.7) 
Multiple polyps:  191 (64.3) 
LocationRight side  129 (43.4) 
Left side  168 (56.6) 
AppearancePedunculated  28 (9.4) 
Sessile  269 (90.6) 
SizePolyps >10mm  66 (22.2) 
Polyps 6–10mm  31 (6.10.4) 
Polyps ≤5mm  200 (67.3) 
Histologic diagnosisHigh-grade dysplastic conventional adenoma  22 (7.4) 
Sessile serrated adenoma  26 (8.8) 
Traditional serrated adenoma  4 (1.3) 
Low-grade dysplastic conventional adenoma  200 (67.3) 
Microvesicular hyperplastic polyp  25 (8.4) 
Globet cell rich hyperplastic polyp  20 (6.7) 

The values for the results of the biomarker tissue immunohistochemical expression compared with clinical-pathological and endoscopic variables are summarized in Table 2.

Table 2.

Comparison of immunoexpression and p values between clinical, endoscopic and anatomopathological data.

      PI3K (%)COX2 (%)PTEN (%)Ki67 (%)
    n  Average  Median  p value  Average  Median  p value  Average  Median  p value  Average  Median  p value 
Previous CaNo  285  1.09  0.42  0.966  8.41  8.84  0.481  2.40  1.88  0.098  43.4  40.0  0.715 
Yes  12  1.03  0.34    7.58  7.81    3.26  2.58    44.2  40.0   
One or multipleOne  106  0.99  0.36  0.640  8.25  8.94  0.936  2.53  2.15  0.567  43.5  40.0  0.862 
Multiple  191  1.14  0.45    8.45  8.80    2.39  1.86    43.4  40.0   
LocationRight  129  1.24  0.53  0.057  8.40  8.84  0.824  2.33  1.98  0.762  47.4  50.0  0.0001 
Left  168  0.97  0.35    8.36  8.78    2.52  1.89    40.3  40.0   
AppearanceSessile  269  1.08  0.38  0.133  8.24  8.77  0.009  2.34  1.84  0.002  43.2  40.0  0.331 
Pedunculated  28  1.18  0.93    9.65  9.63    3.34  3.23    46.7  40.0   
Size≤5mm  200  1.04  0.39  0.081  8.12  8.38  0.005  2.33  1.82  0.123  42.6  40.0  0.348 
6–10mm  31  0.74  0.74    8.25  9.31    2.30  1.89    47.0  40.0   
>10mm  66  1.39  0.56    9.25  9.60    2.83  2.27    446  40.0   
Histologic diagnosisHDA  22  2.08  1.12  0.031  9.61  9.43  0.013  3.17  2.80  0.044  48.4  40.0  0.001 
LDA  200  1.06  0.38    8.34  8.79    2.50  2.06    47.5  50.0   
TSA  0.86  1.03    9.47  9.64    2.57  3.13    22.5  25.0   
MVHP  25  0.67  0.22    8.73  9.16    2.11  1.43    31.4  40.0   
GCHP  20  1.28  0.57    6.42  7.04    2.23  1.87    30.5  30.0   
SSA  26  0.77  0.43    8.62  8.75    1.82  1.31    34.4  40.0   

Significant p values or values with strong statistical trends are underlined and bold faced.

HAD, high-grade dysplastic conventional adenomas; LDA, low-grade dysplastic conventional adenomas; TSA, traditional serrated adenomas; MVHP, microvesicular hyperplastic polyps; GCHP, globet cell rich hyperplastic polyps; SSA, sessile serrated adenomas.

We can observe that Ki67 exhibited higher values in right colon polyps than in left colon polyps and highest tissue immunoexpression occurred in high-grade and low-grade dysplastic conventional adenomas.

PI3K exhibited higher values in high-grade dysplastic conventional adenomas. Highest tissue immunoexpression of COX2 and PTEN were observed in pedunculated polyps and an increasing tissue immunoexpression of COX2 was observed with greater polyp size.

Concerning the histologic diagnosis of Table 2, the comparison between the results of the types of colonic polyps is seen in Fig. 1, in the Box plots.

Fig. 1.
(0.77MB).

Results and some images of immunohistochemical expression of biomarkers. Left column: Box plots representing an average percentage of positive area for PI3K (A), COX2 (B), PTEN (C) and Ki67 (D) biomarkers in HDA, LDA, TSA, MVHP, GCHP and SSA. Significant statistic differences (p value) were: for PI3K: HDA vs LDA (0.008), MVHP (0.001), SSA (0.016); for COX2: GCHP vs HDA (<0.001), LDA (0.003), TSA (0.041), MVHP (0.009), SSA (0.011); for PTEN: HDA vs MVHP (0.039), SSA (0.005). LDA vs SSA (0.008) and for Ki67 HDA vs TSA (<0.001), MVHP (<0.001), GCHP (<0.001), SSA (<0.001), LDA vs TSA (0.001), MVHP (<0.001), GCHP (<0.001), SSA (0.003). Right column: Microphotographs of immunohistochemical expression of PI3K (E) in a SSA, COX2 (F) in a LDA, PTEN (G) in a LDA and Ki67 (H) in a LDA. Magnification 200×. HAD, high-grade dysplastic conventional adenomas; LDA, low-grade dysplastic conventional adenomas; TSA, traditional serrated adenomas; MVHP, microvesicular hyperplastic polyps; GCHP, globet cell rich hyperplastic polyps; SSA, sessile serrated adenomas; PI3K, phosphatidylinositide-3-kinase; COX2, cyclooxygenase-2; PTEN, phosphatase and tensin homolog; Ki 67, mouse monoclonal antibody against a nuclear antigen expressed in human cells during G1, S, G2 and M phases of cell cycle.

A significant p value comparing the immunoreactivity of Ki67, PI3K and PTEN between MVHPs with HDA and SSA with HDA was observed.

The expression of COX2 in GCHP was significantly lower than that found in all other examined polyps.

The most significant results obtained by ROC curve analysis of variables are described below.

The evaluation between HDA and LDA, using the cut point of 0.50% of immunopositivity per HPF for PIK3 antibody, found significant difference (p=0.013). The PIK3 was more frequently higher than 0.50% in HDA when comparing with LDA.

For COX2 antibody, the cut point of 7.5%, showed significant difference between the diagnosis of GCHP and LDA (p=0.041). LDA were more frequently higher than 7.5% when compared to GCHP.

About PTEN, there is significant difference (p=0.045) between MVHP and LDA, using the cut point 1.5%. When PTEN was higher than 1.5%, was more probable the LDA diagnosis.

The cut point of 45% for KI67, showed significance for comparing LDA and the three most common serrated polyps (SSA, MVHP and GCHP), all of them with p value <0.001. When KI67 was higher than 45%, it was more probably LDA.

Discussion

Ki67 immunoexpression was present in all polyp histological types. The highest tissue immunoexpression occurred in HDA and LDA, followed by SSA and hyperplastic polyp (HP).

In this study, Ki67 exhibited higher values in right colon polyps than in left colon polyps. It can be related to the fact that we found more conventional adenomas in the right side (80.6%) than in the left side (69.6%), and this type of polyp got the highest values for Ki67. However, no relevant difference in Ki67 values regarding the colon location was observed in all conventional adenomas from a study of 47 colorectal polyps in a Middle Eastern population.33

Furthermore, some authors7 have observed higher Ki67 expression in serrated lesions and high-grade dysplastic adenomas.33–35 The highest Ki67 expression in more dysplastic polyps may point to greater proliferating activity, as well as to more cells with self-regulation ability loss.30 Thus, the significance of Ki67 activity may be noteworthy even in the evaluation of the potential progression of benign polyps, possibly indicating a different follow-up for patients with these polyp location.

Moreover, the significance of 45% cut point for Ki67, in our results (p<0.001), suggest that this biomarker can be helpful for pathologists in differential diagnosis between LDA and the three most common serrated polyps (SSA, MVHP and GCHP). When Ki67 was higher than 45% was more probably LDA.

In the case of tissue immunoexpression of PI3K, an increase was observed in the right colon compared with the left colon, it was not significant (p=0.057) but had a strong statistical trend. To date, no evaluation of PI3K colorectal polyp tissue immunohistochemical expression has been available in the literature. Moreover, it is known that the PIK3CA gene (a gene that promotes PI3K regulation) mutation occurs more often in right-sided colorectal cancer.36 In this study, higher PI3K tissue immunoexpression in right colon polyps might be related to this gene's higher mutation rates, with consequent alterations in the progression of the polyp's carcinogenesis pathways.

HDA yielded the highest tissue immunoexpression of PI3K. Some authors who evaluated the presence or absence of the PIK3CA mutation gene in 426 colorectal polyps observed that all 4 mutations occurred in tubular-villous conventional adenomas, findings that may or may not be related to the immunoexpression of PI3K.37 The positive PI3K labeling findings in benign polyps in this study may have posttranscriptional influences other than the PIK3CA gene mutation because the latter, as presented in the literature, is less frequent in these polyps and more frequent in colorectal cancer. Moreover, the finding of highest immunolabeling in more dysplastic polyps may be related to tumor progression occurring in benign colon polyps.

It is being proposed that MVHP can be a precursor of SSA and share the same carcinogenic via,38 the serrated pathway to colorectal carcinoma with microsatellite instability (MSI), a different pathway from that is proposed for the majority of conventional adenomas, the chromosomal instability pathway.39

The higher and significant values for PI3K for HDA when comparing with MVHP and SSA in Fig. 1 may be related with PI3K and RAS interaction. As PI3K is being consider one of the main effector pathways of RAS,40 and mutations in proto-oncogenes such as KRAS lead to conventional adenomatous polyp formation,39 the higher expression of PI3K in HDA, may be related with RAS mutation in HDA.

Besides, in a classification of colorectal cancer in five groups,41 taking account molecular, clinical and morphological features, KRAS mutation is more related with chromosomal instability pathway and is originated more frequently in conventional adenomas.

In addition to, our results also raise the possibility of the use of PI3K antibody for pathological graduation of conventional adenomas. By using the cut point of 0.50% we found significance comparing HDA and LDA (p=0.013). HDA is more probable when PI3K is higher than 0.50%.

Pedunculated polyps in the study were also observed to be larger in size, which may explain the higher values of COX2 because this protein increases during polyp tumor progression.42

Some authors have already reported these findings concerning COX2, with higher COX2 values presenting as directly proportional to the diameter of the polyps.43 These findings also suggest that COX2 may perform a significant role in colorectal cancer promotion and development through the adenoma-carcinoma pathway. Therefore, the use of COX2 inhibitors, such as aspirin, in colorectal cancer prevention through this pathway43–45 would be important. Thus, this finding may help facilitate the development of an effective drug therapy.

A significant higher tissue immunoexpression of COX2 was observed in HDA. Investigators who studied 212 polyps from 175 patients noted that almost 90% of the high-grade dysplastic polyps presented high levels of COX2 labeling, whereas almost 50% of low-grade dysplastic polyps were positive for COX2 and only 24% of polyps with no dysplasia were positive for the same label. Villous histology also presented higher labeling in this series.43

The lower value of COX2 in GCHP comparing to all others polyps was different of the others authors results,46 but in their comparison, the HP were not classified in MVHP, GCHP and MPHP.

Furthermore, for COX2 the cut point of 7.5% was significant when comparing LDA with GCHP, suggesting its use to help this differentiation. It is more probably LDA when COX2 is >7.5%. These lesions sometimes can be difficult to differentiate by pathologists, mainly when there is a disorientation of the lesion at the cut, or the specimen has artifacts, or the specimen is small.

Regarding PTEN, higher tissue immunoexpression was observed for HDA and TSA, followed by LDA and lower immunoexpression for others serrated lesions (GCHP, MVHP and SSA). The literature supports progressive PTEN expression loss in colorectal carcinogenesis stages with the lowest expression in malignant lesions47; however, differences between polyp types were not reported.48 The colorectal tumorigenesis process is usually slow, with adenomas appearing usually 10 to 15 years prior to colorectal adenocarcinomas.49 How PTEN behaves at critical tumorigenesis stages is unknown. The major findings in this study concerning PTEN immunoexpression in more cytological dysplastic polyps may signify a containment attempt regarding the progression of this lesion tumor type. The lowest expression of PTEN found in this study was in GCHP, MVHP and SSA might be related to an expression suppression of this molecule and consequently, a more rapid carcinogenesis pathway in this type of polyp. Moreover, it may indirectly represent a possible apoptosis loss in these polyps, a finding that has already been described for conventional adenomas.47

The main limitations of this study refer to the fact that this is a retrospective study using immunohistochemistry evaluation. Further studies with colon polyps, using molecular biology techniques could help in understanding the carcinogenesis of colon cancer.

Conclusions

The results support the observation that lesions with more severe dysplasia tend to have higher values of Ki67, which appears to coincide with higher values of PTEN, perhaps reflecting an attempt to control the cellular cycle and consequently, tumorigenesis. The higher values in these polyps of PI3K and COX2 data support the hypothesis that both increase while colonic polyp dysplasia increases. In this series of 297 benign colon polyps, Ki67 and PI3K presented higher tissue immunoexpression in the right colon, which may suggest a different carcinogenesis pathway in this colonic segment. Pedunculated polyps presented higher COX2 and PTEN tissue expression, which may be related, in the case of COX2, to tumor progression, and with respect to PTEN, to an attempted containment of the same tumor progression. COX2 presented the highest values in larger polyps and in polyps with higher-grade dysplasia. PI3K presented highest tissue immunoexpression in the most dysplastic polyp histologic type. PTEN presented higher labeling in high-grade dysplastic conventional adenomas, which may signify an attempted containment of tumor progression. Moreover, PTEN presented lower labeling values in sessile serrated adenomas, which may be related to greater apoptosis loss, with more rapid tumor progression in these polyps.

Conflicts of interest

The authors declare no conflicts of interest.

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Study conducted at Pontifícia Universidade Católica do Paraná (PUCPR), Faculdade de Medicina, Curitiba, PR, Brazil.

Corresponding author. (Rosimeri Kuhl Svoboda Baldin rosimeribaldin@gmail.com)
Copyright © 2017. Sociedade Brasileira de Coloproctologia
J Coloproctol. (Rio J.) 2018;38:1-8 - Vol. 38 Num.1 DOI: 10.1016/j.jcol.2017.08.005